The Occurrence of Folate Biosynthesis Genes in Lactic Acid Bacteria from Different Sources

Research background. Lactic acid bacteria (LAB) are known to produce folate. However, this ability is highly strain-dependent. Folate synthesis in specific LAB strains affected by the availability of folate, which can be consumed other under certain conditions. Moreover, differences folate capabilities related presence biosynthesis-related genes and regulation pathway. Experimental approach. As basic information better understand biosynthesis among different species strains, biosynthetic were screened identified folate-producing non-folate-producing isolated from various local food sources Indonesia. The extracellular productivity amounts isolates analyzed using high-performance liquid chromatography with a diode array detector (HPLC-DAD). Results conclusions. Eleven thirteen tested had all eight involved (folE, folQ, folB, folK, folP, folC1, folA folC2). Furthermore, these produced ranging 10.37 31.10 μg/mL. In contrast, two lacked several genes, such as folP folA, possibly reason for their inability synthesize de novo. Phylogenetic tree construction revealed that (excluding folK folP) six distinct monophyletic homologous database. Novelty scientific contribution. study, distribution was determined. findings research support use marker genotypic screening LAB.